Synthesis and in vitro Cytotoxicity Testing of New Cyclen-Peptide Conjugations

Abstract

the new small peptide functionalized cyclen (1,4,7,10-tetraazacyclododecan) and L-DOPA (3,4-dihydroxyphenylalanine)
derivatives were synthesized: cyclen-HisHis, cyclen-AspHis, cyclen-GluHis, DOPA-HisHis, as well as their Cu(II) and/
or Zn(II) coordination compounds were prepared. The solid-phase synthesis strategy was used for preparation of new
compounds. Synthesized cyclen- and DOPA-oligopeptide hybrid conjugations were purified by HPLC and analyzed
using MS-ES spectrometer. The cytotoxicity assay showed that cyclen-dipeptide hybrids are non-toxic for cell line Hep
G2 - ATCC® HB-8065TM (cells are derived from human liver) and HEK-293T - ATCC® CRL-11268TM (epithelial cells
derived from kidney of human fetus).The antioxidant and anticancer activity studies will be next part of the ongoing
project.